Shiraz E-Medical Journal

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Enzymatic Characterization of Acid Phosphatase in the Logarithmic and Stationary Phase of Leishmania major Promastigotes

Amir Navabi 1 and Simindokht Soleimanifard 2 , *
Authors Information
1 Alzahra Medical Center, Department of Parasitology, Isfahan University of Medical Sciences, Isfahan, IR Iran
2 Department of Parasitology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran
Article information
  • Shiraz E-Medical Journal: January 01, 2015, 16 (1); e26246
  • Published Online: January 19, 2015
  • Article Type: Research Article
  • Received: December 20, 2014
  • Accepted: December 22, 2014
  • DOI: 10.17795/semj26246

To Cite: Navabi A, Soleimanifard S. Enzymatic Characterization of Acid Phosphatase in the Logarithmic and Stationary Phase of Leishmania major Promastigotes, Shiraz E-Med J. 2015 ; 16(1):e26246. doi: 10.17795/semj26246.

Copyright © 2015, Shiraz University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Alvar J, Velez ID, Bern C, Herrero M, Desjeux P, Cano J, et al. Leishmaniasis worldwide and global estimates of its incidence. PLoS One. 2012; 7(5)[DOI][PubMed]
  • 2. do Monte-Neto RL, Coelho AC, Raymond F, Legare D, Corbeil J, Melo MN, et al. Gene expression profiling and molecular characterization of antimony resistance in Leishmania amazonensis. PLoS Negl Trop Dis. 2011; 5(5)[DOI][PubMed]
  • 3. Rogers ME, Chance ML, Bates PA. The role of promastigote secretory gel in the origin and transmission of the infective stage of Leishmania mexicana by the sandfly Lutzomyia longipalpis. Parasitology. 2002; 124: 495-507[PubMed]
  • 4. Mojtahedi Z, Clos J, Kamali-Sarvestani E. Leishmania major: identification of developmentally regulated proteins in procyclic and metacyclic promastigotes. Exp Parasitol. 2008; 119(3): 422-9[DOI][PubMed]
  • 5. Pimenta PF, Saraiva EM, Sacks DL. The comparative fine structure and surface glycoconjugate expression of three life stages of Leishmania major. Exp Parasitol. 1991; 72(2): 191-204[PubMed]
  • 6. Bandyopadhyay P, Ghosh DK, De A, Ghosh KN, Chaudhuri PP, Das P, et al. Metacyclogenesis of Leishmania spp: species-specific in vitro transformation, complement resistance, and cell surface carbohydrate and protein profiles. J Parasitol. 1991; 77(3): 411-6[PubMed]
  • 7. Isnard A, Shio MT, Olivier M. Impact of Leishmania metalloprotease GP63 on macrophage signaling. Front Cell Infect Microbiol. 2012; 2: 72[DOI][PubMed]
  • 8. Fernandes AC, Soares DC, Saraiva EM, Meyer-Fernandes JR, Souto-Padron T. Different secreted phosphatase activities in Leishmania amazonensis. FEMS Microbiol Lett. 2013; 340(2): 117-28[DOI][PubMed]
  • 9. Baghaei M, BAGHAEI M. Characterization of acid phosphatase in the promastigotes of three isolates of Leishmania major. Iran J Med Sci. 2003; 28(1): 1-8
  • 10. The role of energy metabolism enzymes in physiopathology of Cutaneous Leishmaniasis. Thirteen congress of physiology & pharmacology.
  • 11. Bogdan C, Rollinghoff M. The immune response to Leishmania: mechanisms of parasite control and evasion. Int J Parasitol. 1998; 28(1): 121-34[PubMed]
  • 12. Rezazadeh Marznaki F, Shakeri R, Kaboudanian Ardestani S, Tahghighi A, Foroumadi A. In vitro immunobiological studies of novel 5-(5-nitrofuran-2-yl)-1, 3, 4-thiadiazoles with piperazinyl-linked benzamidine substituents against Leishmania major. Iran J Allergy Asthma Immunol. 2013; 12(4): 368-76[PubMed]
  • 13. Sarkari B, Chance M, Hommel M. Antigenuria in visceral leishmaniasis: detection and partial characterisation of a carbohydrate antigen. Acta Trop. 2002; 82(3): 339-48[PubMed]
  • 14. Ilg T. Lipophosphoglycan is not required for infection of macrophages or mice by Leishmania mexicana. EMBO J. 2000; 19(9): 1953-62[DOI][PubMed]
  • 15. Ghosh S, Goswami S, Adhya S. Role of superoxide dismutase in survival of Leishmania within the macrophage. Biochem J. 2003; 369: 447-52[DOI][PubMed]
  • 16. Shio MT, Olivier M. Editorial: Leishmania survival mechanisms: the role of host phosphatases. J Leukoc Biol. 2010; 88(1): 1-3[DOI][PubMed]
  • 17. Turco SJ, Spath GF, Beverley SM. Is lipophosphoglycan a virulence factor? A surprising diversity between Leishmania species. Trends Parasitol. 2001; 17(5): 223-6[PubMed]
  • 18. Martiny A, Meyer-Fernandes JR, de Souza W, Vannier-Santos MA. Altered tyrosine phosphorylation of ERK1 MAP kinase and other macrophage molecules caused by Leishmania amastigotes. Mol Biochem Parasitol. 1999; 102(1): 1-12[PubMed]
  • 19. Baghaei M, Mesripour M. Leishmania enzyme activity in the promastigote of L.tropica and L.major. Iran J Med sci. 1996; 21(182): 11-5
  • 20. Baghaei M, Mesripour M. Differences in enzyme activity and biology behavior of three leishmania promastigotes isolated from patient with zoonotic cutaneous leishmaniasis. Irav J Med sci. 1974; 22(182): 7-14
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