A Rapid Modified Antibody-Capture Enzyme-Linked Immunosorbent Assay (ELISA) for Detection HSV-1 Primary Infection in Human

AUTHORS

M Ravanshad * , +

How to Cite: Ravanshad M. A Rapid Modified Antibody-Capture Enzyme-Linked Immunosorbent Assay (ELISA) for Detection HSV-1 Primary Infection in Human, Shiraz E-Med J. Online ahead of Print ; 7(2):20366.

ARTICLE INFORMATION

Shiraz E-Medical Journal: 7 (2); 1-6
Published Online: April 1, 2006
Article Type: Research Article

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Abstract

An antibody-capture enzyme-linked immunosorbent assay (ELISA) based on the reaction between anti human IgM and serum IgM and HSV-1 conjugated with horseradish peroxidase (HRP) was developed. The specificity and sensitivity of the developed HSV-1 IgM ELISA were evaluated and compared with a commercially available test kit by using sets of well defined sera of known HSV-1 primary infection. For validation, 526 serum samples have been tested. The HSV-1 IgM ELISA had acceptable sensitivity and specificity, which were comparable with that of the commercial test kit. Both assays showed some cross-reactivity with sera containing anti-HSV-2 antibodies

Keywords

Herpes Simplex Virus, ELISA, IgM

© 2006, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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