Cite Score:
0.65
ELSEVIER SCOPUS

Typing of Aspergillus Fumigatus and Aspergillus Niger Strains by Random Amplification of Polymorphic DNA Analysis Using a Six Primer Set.

AUTHORS

H Mirhendi 1 , * , M Moazeni 2 , M Nikaeen 3 , K Makimura 4

AUTHORS INFORMATION

1 Associate Professor, Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Researches, Tehran University of Medical Sciences, Tehran, Iran,

2 PhD Student of Medical Mycology, Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Researches, Tehran University of Medical Sciences, Tehran, Iran,

3 Assistance professor, Department of Environmental Health Engineering, Isfahan University of Medical Sciences, Isfahan, Iran,

4 Professor, Institute of Medical Mycology and Genome Research Center, Teikyo University, Tokyo, Japan.

ARTICLE INFORMATION

Shiraz E-Medical Journal: 10 (4); 190-200
Published Online: October 1, 2009
Article Type: Research Article
Received: February 3, 2009
Accepted: May 4, 2009

Crossmark

CHEKING

READ FULL TEXT
Abstract

Abstract: Differentiating and typing clinical and environmental isolates of Aspergillus strains are necessary for epidemiological studies and could contribute to the solution of several pertinent clinical problems. We investigated the utility of the RAPD-PCR technique for typing twenty-six strains of Aspergillus fumigatus and ten additional clinical and environmental Aspergillus fumigatus and Aspergillus niger isolates. Fungal genomic DNA was extracted using glass bead disruption and RAPD analysis was performed by using a six individual (R108, R151, UBC90) and combined (R108+R151, R108+UBS90, R151+UBC90) primer set. Primer pair R108+UBC90 demonstrated the highest discriminatory power with collection strains, whereas primer R151 displayed the highest degree of discrimination power with clinical and environmental isolates. Both primer sets detected seven types of strains. It was found that primer R151, on its own and in combination with primer UBC90, shared a relative relationship between clinical and environmental A. fumigatus isolates. We found that although RAPD-PCR analysis can be applied as a simple, rapid, and very effective method for differentiating Aspergillus strains, selecting the best primers is a critical step to reach the desirable discriminatory power.

Keywords

Aspergillus

© 2009, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
Full Text

Full text is available in PDF

COMMENTS

LEAVE A COMMENT HERE: